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		<citationkey>SilvaLeitStol:2017:CoMuMi</citationkey>
		<title>Combining Multifocal Microscopy and Photometric Stereo with a Low Cost Motorized Positioner</title>
		<format>On-line</format>
		<year>2017</year>
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		<author>Silva, Gustavo Lelis da,</author>
		<author>Leitão, Helena Cristina da Gama,</author>
		<author>Stolfi, Jorge,</author>
		<affiliation>UFF</affiliation>
		<affiliation>UFF</affiliation>
		<affiliation>Unicamp</affiliation>
		<editor>Torchelsen, Rafael Piccin,</editor>
		<editor>Nascimento, Erickson Rangel do,</editor>
		<editor>Panozzo, Daniele,</editor>
		<editor>Liu, Zicheng,</editor>
		<editor>Farias, Mylène,</editor>
		<editor>Viera, Thales,</editor>
		<editor>Sacht, Leonardo,</editor>
		<editor>Ferreira, Nivan,</editor>
		<editor>Comba, João Luiz Dihl,</editor>
		<editor>Hirata, Nina,</editor>
		<editor>Schiavon Porto, Marcelo,</editor>
		<editor>Vital, Creto,</editor>
		<editor>Pagot, Christian Azambuja,</editor>
		<editor>Petronetto, Fabiano,</editor>
		<editor>Clua, Esteban,</editor>
		<editor>Cardeal, Flávio,</editor>
		<e-mailaddress>hcgl@ic.uff.br</e-mailaddress>
		<conferencename>Conference on Graphics, Patterns and Images, 30 (SIBGRAPI)</conferencename>
		<conferencelocation>Niterói, RJ, Brazil</conferencelocation>
		<date>17-20 Oct. 2017</date>
		<publisher>Sociedade Brasileira de Computação</publisher>
		<publisheraddress>Porto Alegre</publisheraddress>
		<booktitle>Proceedings</booktitle>
		<tertiarytype>Industry Application Paper</tertiarytype>
		<transferableflag>1</transferableflag>
		<keywords>Multifocal Microscopy, Photometric Stereo, Multifocus image, 3D Printer.</keywords>
		<abstract>Multifocal microscopy is an established technique to combine several reflected-light microscopy images of an object, each with a limited depth of focus, into a single image that shows the whole object in focus.  Photometric stereo is an independent technique to recover the third dimension of an opaque object, given several images taken from the same viewpoint with different illumination conditions.  We describe here the synergistic combination of both techniques to obtain accurate three-dimensional images of microscopic objects.  In order to streamline the acquisition of the necessary images, we developed a low-cost positioner that automatically varies the microscope-to-object distance and the light direction, under control of an Arduino microcontroller.  The main structural parts of the support were manufactured by a 3D printer.   The technique and support are demonstrated with a low cost digital microscope.</abstract>
		<language>en</language>
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		<usergroup>hcgl@ic.uff.br</usergroup>
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